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COORDINATOR
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Project
under NRRI
2020-2027
"National
Center of
Biomedical
Photonics-NCBP"
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PROJECTS
PROJECT
#
KP06-India-8 /
07.08.2019 |
Project
presentations |
Project
publications |
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# KP06-India-8
/ 07.08.2019
„Polarization-sensitive
fluorescence
spectroscopy
and microscopy
for cancer
detection“
Funded by
NSF-MES-Bulgaria
- Partners:
- Institute
of Electronics,
Bulgarian
Academy of
Sciences
- Department
of Biophysics,
School of Life
Sciences,
Manipal Academy
of Higher
Education,
Manipal,
Karnataka,
India
-
-
-
Institute
of Electronics,
BAS
Department
of Biophysics,
School of Life
Sciences,
Manipal Academy
of Higher
Education,
Manipal,
Karnataka,
India
-
-
-
Bulgarian
scientific team
- Ekaterina
Borisova,
Assoc. prof.,
PhD, IE-BAS –
team
coordinator
- Latchezar
Avramov,
Prof., D. Sci.,
PhD, IE-BAS
- Alexander
Gisbrecht,
Assoc. prof.,
PhD, IE-BAS
- Tsanislava
Genova, PhD
student, IE-BAS
- Deyan
Ivanov, PhD
student, IE-BAS
- Stoyan
Ilyov, BS
student in
“Medical
physics”, SU,
technician –
IE-BAS
-
-
Indian
scientific team
- Dr.
Nirmal Mazumder,
Assistant
Professor,
Ph.D. – team
coordinator
- Shama
Parsada
Kabekkodu,
Associate
Professor, PhD
- Sindhoora
K M, Ph.D.
Scholar, MSc
PROJECT
SUMMARY
Modern methods
for the study
of collagen and
elastin fibers
in biological
tissues require
significant
sample
processing and
are limited to
the methods of
histology and
immunohistochemistry.
Stokes -
polarimetry on
the other hand,
based on
polarization
multimodal
imaging and
integrated with
second harmonic
generation
(SHG)
microscopy is
one of the
newest and most
studied areas
of biophotonics
worldwide, due
to the
prospects of
obtaining
information on
dynamic
cellular and
tissue changes
in vivo, the
technique
allowing the
measurement of
the four Stokes
vectors
simultaneously,
using a
4-detector
scheme.
Simultaneous
measurement is
an important
factor in
characterizing
the overall
state of
polarization
and thus
improving the
sensitivity and
minimizing
instrumental
errors due to
background
noise. The
visualization
of fibrous
tissue
structures is
difficult when
applying
methods for
obtaining
conventional
polarization
images, but is
significantly
similar when
applying the
method for
obtaining
vector Stokes
images.
However, the
high cost and
complexity of
the equipment
used for this
type of
measurement
limits its
applications.
The high
sensitivity of
fluorescence
spectroscopy
allows it to be
used as a tool
for early
diagnosis of
pathological
changes in
tissues at the
biochemical
level before
the onset of
obvious
morphological
changes. The
combination
with
polarization
techniques for
autofluorescence
spectroscopy of
skin neoplasms
will contribute
to obtaining
information
about the
anisotropic
properties of
skin tissues
and their
changes during
tumor growth.
Polarization
will improve
the optical
analysis of
morphological
changes to
assess the
anisotropic
changes of
structural
proteins in the
extracellular
matrix during
the development
of neoplasia,
which can be
used not only
to assess
malignancies
but also to
detect
degenerative
changes in
connective
tissues,
autoimmune
diseases such
as lupus
erythematosus,
dermatomyositis,
scleroderma,
rheumatoid
arthritis and
others.
The
combination of
previous
research,
theoretical,
experimental
and clinical
experience of
the two
research
groups, the
accumulation of
new
experimental
data and the
development of
spectral and
imaging
algorithms in
the framework
of joint
cooperation
will form the
basis of this
project,
helping to
develop and
implement
combined
optical
techniques for
detection and
differentiation
of skin
neoplasms and
other systemic
skin diseases,
for
optimization
and upgrading
with
polarization-sensitive
channels, etc.
the spectral
techniques we
use in our
research
practice and to
enrich the
instrumental
possibilities
for
polarization-sensitive
spectral
diagnostics of
tissue
pathologies in
order to
increase the
diagnostic
accuracy for
clinical
applications
and to develop
a methodology
for
polarization-sensitive
fluorescence
spectroscopy
and microscopy
for cancer
detection.
PROJECT
OBJECTIVES
The main aim of
this project is
to determine
the
polarization
emission
properties of
healthy and
diseased
tissues using
modern optical
methods. The
Bulgarian and
Indian groups
aim to evaluate
the potential
of
polarization-sensitive
autofluorescence
spectroscopy
and
Stokes-Mueller
microscopy of
biological
tissues and
thus to develop
a new objective
tool for
biomedical
applications.
In this
context, we
propose to
create a common
research
platform to
analyze and
evaluate the
polarization
properties of
the emitted
light under
fluorescent
excitation
based on our
expertise.
The
main objectives
of the project
proposal are
the following:
- Visual
assessment and
quantification
of the
polarization
characteristics
of the tissue
(collagen
fibers,
elastin), using
autofluorescence
spectroscopy
and microscopy;
- Determination
of the
correlations of
the
polarization
properties of
healthy tissues
and their
changes during
tumor growth,
autofluorescence
spectroscopy
with
cross-polarization
and
Stokes-vector
autofluorescence
microscopy;
- Segmentation
of images,
reconstruction
and automation
of the
differentiation
of healthy and
tumor skin
tissues;
- Creation
and development
of fruitful
scientific
cooperation, as
the results of
the research
will be
described in
joint
publications in
peer-reviewed
journals and
presented at
prestigious
international
conferences;
supporting the
career
development of
young
scientists and
PhD students in
the project
teams of both
partners.
-
-
-
Presentations
under #
KP06-India-8 /
07.08.2019
“Polarization-sensitive
fluorescence
spectroscopy
and microscopy
for cancer
detection”:
- Ivanov
Deyan, Borisova
Ekaterina,
Ossikovski
Razvigor, Bykov
Alexander,
Dremin Victor,
A full Mueller
matrix
measurement of
ex vivo colon
samples with
Stokes
polarimeter, V
Summer School
"Photonics meet
Biology",
16.09.2019 -
20.09.2019,
Heraklion,
Greece, (Poster
presentation);
- Ilyov
S., Ivanov D.,
Genova Ts.,
Mircheva V.,
Zaharieva L.,
Kolev B.,
Vladimirov, B.,
Valkov H.,
Mazumder N.,
Sindhoora K.,
Semyachkina-Glushkovskaya
O., Borisova
E., Reflectance
polarization
measurements of
gastrointestinal
carcinoma
lesions ex vivo
for cancerous
diagnostics,
21st
International
Conference and
School on
Quantum
Electronics
"Laser Physics
and
Applications",
21.09.2020 -
24.09.2020,
Sofia, Bulgaria
(virtual forum)
(Poster
presentation);
- Ivanov
D., Borisova
E., Novikova
T., Ossikovski
R. Experimental
validation of
depolarizing
Mueller matrix
model via ex
vivo colon
samples, 21st
International
Conference and
School on
Quantum
Electronics
"Laser Physics
and
Applications",
21.09.2020 -
24.09.2020,
Sofia, Bulgaria
(virtual forum)
(Poster
presentation);
- Sindhoora
K., Spandana
K., Ivanov D.,
Borisova E.,
Raghavendra U.,
Rai Sh.,
Kabekkodu S.
P., Mahato K.,
Mazumder N.,
Machine
Learning Based
Classification
of
Stokes-Mueller
Polarization
Images for
Tissue
Characterization
21st
International
Conference and
School on
Quantum
Electronics
"Laser Physics
and
Applications",
21.09.2020 -
24.09.2020,
Sofia, Bulgaria
(virtual forum)
(Poster
presentation);
Publications
under
KP06-India-8 /
07.08.2019
“Polarization-sensitive
fluorescence
spectroscopy
and microscopy
for cancer
detection”:
-
Ilyov S.,
Ivanov D.,
Genova Ts.,
Mircheva V.,
Zaharieva L.,
Kolev B.,
Vladimirov, B.,
Valkov H.,
Mazumber N.,
Sindhoora K.,
Semyachkina-Glushkovskaya
O., Avramov L.,
Borisova E..
Reflectance
Polarization
Measurements of
Gastrointestinal
Carcinoma
Lesions Ex Vivo
for Cancerous
Diagnostics.
Journal of
Physics:
Conference
Series, IOP
Publishing Ltd.
2020, SJR=0.23
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